荠菜ＣＢＦ途径关键基因ＣｂＣＢＦ的启动子及其应用制造技术

本发明专利技术属于植物基因工程技术领域，具体涉及一种荠菜ＣＢＦ途径关键基因ＣｂＣＢＦ的启动子ＣＢＦＰ及其应用。该启动子的核苷酸序列如ＳＥＱ？ＩＤ？ＮＯ：１所示，其中ＣｂＣＢＦ基因及其启动子包含序列表ＳＥＱ？ＩＤ？ＮＯ：１所示序列中的２２８３位碱基的核酸序列，该核酸序列编码一个受低温诱导的、能调控冷诱导基因的转录因子。启动子区域包含两个串联排列的ＡＢＲＥ顺式作用元件，能特异地对低温和ＡＢＡ起应答反应，和三个ＭＹＣＲＥ顺式作用元件ＣＡＮＮＴＧ，能与上游调控因子ＩＣＥ（ｉｎｄｕｃｅｒ？ｏｆ？ＣＢＦ？ｅｘｐｒｅｓｓｉｏｎ）基因结合。利用该基因启动子构建的诱导性表达载体可以在植物受到低温胁迫时强烈地诱导目标基因的表达。本发明专利技术还提供上述启动子在培育抗寒植物中的应用，实现农作物品种的改良。

Key genes CbCBF CBF pathway Capsella promoter and its application

The invention belongs to the technical field of plant gene engineering, in particular to a promoter CBFP CBF pathway gene CbCBF pickpurse and its application. The nucleotide sequence of the promoter, such as SEQ? ID? NO:1 shows that the CbCBF gene and its promoter contain the sequence table SEQ ID? The nucleotide sequence of the 2283 base nucleotide in the sequence shown in NO:1, which encodes a transcription factor that is low temperature inducible and can regulate cold induced genes. The promoter region contains two tandemly arranged ABRE cis element specific response to low temperature and ABA, and three MYCRE CANNTG cis element, and upstream regulatory factor ICE (inducer? Of? CBF? Expression) gene binding. The inducible expression vector constructed by the gene promoter can induce the expression of target genes when plants are subjected to low temperature stress. The invention also provides the application of the promoter in the cultivation of cold resistant plants and realizes the improvement of crop varieties.

【技术实现步骤摘要】

【技术保护点】
一种分离的具有低温诱导表达特性的荠菜启动子，其特征在于该启动子的核苷酸序列如ＳＥＱ　ＩＤ　ＮＯ：１中碱基第１－１６２３位的序列所示，记为ＣｂＣＢＦＰ。

【技术特征摘要】

【专利技术属性】
技术研发人员：林娟，周明琦，吴丽华，
申请(专利权)人：复旦大学，
类型：发明
国别省市：31[]