The present invention discloses Eimeria tenella (Eimeria tenella) is a new protein kinase (EtPK) gene, clone: ZB1 - A02, the length of 1684bp, contains a 1527bp ORF encoding 508 amino acids (Genbank accession No. EF552216). In the invention, the gene was cloned into the prokaryotic expression vector pGEX - 4T - 2, prokaryotic expression plasmid pGEX - 4T - EtPK, and expressed in Escherichia coli, the recombinant protein existed in the form of inclusion body. The purified recombinant protein 4T - EtPK SDS - PAGE, and then transferred to PVDF membrane, using antiserum E.tenella oocysts as anti - oral immunization of chicken, Goat anti chicken IgG as the two antibody in Western blot analysis show that it has certain antigenicity and can be used for the development of anti coccidiosis medicine and preparation of chicken coccidiosis vaccine.
【技术实现步骤摘要】
【技术保护点】
一种柔嫩艾美耳球虫蛋白激酶基因，其特征在于所述该柔嫩艾美耳球虫蛋白激酶基因具有下列序列： ＡＧＡＡＣＧＡＧＴＴＧＴＴＧＣＣＡＡＴＧＧＡＧＡＧＴＣＣＡＣＣＡＧＴＡＡＧＣＴＴＴＣＣＣＧＡＧＧＣＡＧＴＧＣＣＴＧＧＴＧＣＧＴＡＣＴＣＣＧＡＡＡ ＧＴＧＣＴＡＡＧ ＧＣＣＣＣＴＡＴＡＧＡＧＡＡＧＣＣＡＡＡＧＡＡＣＡＡＡＧＴＡＡＣＡＡＡＣＧＡＡＧＣＡＧＡＧＧＧＡＧＡＴＧＡＣＡＧＣＡＡＴＧＣＴＴＴＣＴＴＣＣＴＡＡＡＣＧＣＧＣＧ ＧＣＣＴＧＧＣＣＧＡＣＧＧＣＡＧＣＡＧＣＧＧＣＡ ＡＧＴＴＧＧＣＴＣＧＣＧＣＧＴＴＧＣＴＣＴＧＧＧＴＴＴＴＧＧＣＣＴＧＡＴＧＴＴＴＴＣＣＧＧＣＣＴＴＧＴＴＧＣＡＧ ＣＴＡＴＣＣＴＡＴＣＴＡＧＣＣＴＧＣＴＧＴＴＧＣＧＧＣＡＧＣＴＣＣＡＡＧＡＡＣＣＴＣＣＣＣＧＡＧＴＣＡＡＣＣＴＧＴＣＣＡ ＡＣＧＣＡＴＧＧＣＡＧＣＡＧＣＴＧＧＡＧ ＴＣＧＣＡＧＧＡＧＧＣＧＡＣＧＴＧＧＣＴＧＣＴＴＣＣＴＡＣＴＧＴＡＧＡＣＣＡＧＣＡＡＧＡＡＧＴＡＧＣＧＧＣＴＣＡＣＣＣＴＣＧＣＴＴＧＧＣＣＡＴＧＧＧＴＡＡＡＴＧ ＧＴＴＧＣＴＴＧＣＡＧＡ ＴＧ...
【技术特征摘要】
【专利技术属性】
技术研发人员：韩红玉，黄兵，林矫矫，赵其平，董辉，姜连连，颜彦，姚倩，樊玉娟，李玉剑，
申请(专利权)人：中国农业科学院上海兽医研究所，
类型：发明
国别省市：31[中国|上海]
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