The invention relates to a method for improving the survival rate of a salt birch seed, belonging to the technical field of plant tissue culture and seedling transplanting, in particular to the tissue culture and propagation technique of the salt birch and the seedling transplanting technique of the test tube seedling. Its characteristic is composed of betulahalophila tissue culture and transplanting two processes, and respectively according to the operation steps of the implementation of betulahalophila tissue culture and transplanting: production of sterile buds, proliferation, rooting and seedling transplanting, pretreatment, making culture medium, after transplanting and management. The invention improves the survival rate of salt birch seed seedlings, and the transplanting survival rate is more than 95%. In order to produce a large quantity of high-quality birch seedlings, it is necessary to meet the needs of improving the soil and greening in the saline alkali soil of Lingang New Town in Shanghai.
【技术实现步骤摘要】
属于植物组织培养,炼苗移栽
,确切地说是涉及盐桦的组织培养繁殖技术和试管苗炼苗移栽技术。
技术介绍
盐桦(Betrla halophila Ching)为桦木科桦木属植物,落叶小乔木或直立大灌木,树高3 4米。树皮和小枝为灰褐色,小枝有短而密的白色柔毛,及黄色的树脂腺体。叶长2.5 4.5厘米,卵形或菱形。果序为下垂的圆柱形,长2 3厘米,直径约l厘米。小坚果呈卵形,长2毫米,宽1. 5毫米,膜质果翅宽阔,宽度为果的1. 5倍,翅的两面均有疏而短的柔毛。属于中性植物,喜光,喜土壤潮湿。它的地理分布范围极为狭小,仅产于阿勒泰县内朗河的下游,是我国著名的植物学家秦仁昌教授发现定名的新种。 盐桦抗盐能力非常强,超过世界上任何一种乔木,在幼苗期抗盐率即可达到1. 3%以上,是以抗盐碱著称的胡杨、沙枣抗盐率的5倍以上,是盐碱地绿化和造林的重要树种,也可以改良土壤,因此其保护和开发价值很大。它被列为国家二级珍稀濒危植物,是原生地已濒临灭绝的小乔木树种,仅存于我国新疆阿勒泰地区, 一旦灭绝,则我国乃至世界植物名录上将永远消失。因此开展盐桦繁殖方面的研究,对推广和应用盐桦 ...
【技术保护点】
一种提高盐桦苗种成活率的方法,其特征是:由盐桦组织培养和炼苗移栽两个过程构成,并按以下操作步骤分别实施盐桦组织培养和炼苗移栽: (1)制作无菌芽:在生长季节,选用半木质化的带芽茎段作为外植体,用洗洁精搓洗去枝条表面的毛;以70%的乙醇浸泡1min;后以10%次氯酸钠浸泡12min;灭菌处理后,将茎段接种到培养基MS+BA1.0~1.5mg/L进行培养; (2)增殖过程:将无菌芽接种于培养基MS+6-BA0.1-1.0mg/L+NAA0-0.5mg/L中,并每隔4周将新芽和茎段分割切下并接种到新的培养基中继续扩增; (3)壮苗和生根:将无菌芽接种到MS+BA ...
【技术特征摘要】
【专利技术属性】
技术研发人员:崔心红,罗玉兰,朱义,张群,
申请(专利权)人:上海市园林科学研究所,
类型:发明
国别省市:31[中国|上海]
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