一种结核分枝杆菌蛋白组、其筛选方法及其应用技术

本发明专利技术公开了一种结核分枝杆菌蛋白组、其筛选方法及其应用；结核分枝杆菌蛋白组的筛选方法包括将人T细胞蛋白和结核分枝杆菌全蛋白质组芯片进行杂交，选择呈阳性位点的结核分枝杆菌蛋白，得到结核分枝杆菌蛋白组；结核分枝杆菌蛋白组用于制备与结核分枝杆菌相关的检测试剂、制备结核治疗试剂、研究结核分枝杆菌的作用机制等；结核分枝杆菌蛋白组，对于结核病的诊断、治疗，特别是结核分枝杆菌的发病机制和开发结核疫苗的研究提供潜在的靶点。制和开发结核疫苗的研究提供潜在的靶点。制和开发结核疫苗的研究提供潜在的靶点。

【技术实现步骤摘要】
一种结核分枝杆菌蛋白组、其筛选方法及其应用


[0001]本专利技术涉及一种结核分枝杆菌蛋白组、其筛选方法及其应用，属于生物学


技术介绍

[0002]结核分枝杆菌(Mycobacterium tuberculosis，Mtb)感染引起的结核病(Tu berculosis，TB)是一种慢性消耗性传染病，已成为威胁人类健康的全球性问题。WHO发布的《2021年全球结核病报告》显示，2020年，全球结核潜伏感染人群接近20亿，新发结核病患者987万，发病率为127/10万，估算发病数和发病率持续呈现下降趋势，但下降幅度较往年有所减缓。排除新冠肺炎疫情的影响，结核病曾是单一传染源的头号死亡原因，也是全球第13大死因。近年来，耐药结核病尤其是耐多药结核病(multidrug
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resistant tuberculosis，MDR
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TB)和广泛耐药结核病(extensively drug
‑
resistant tuberculosis，XDR
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TB)的出现，以及结核分枝杆菌耐药率的持续升高，给抗结核化学治疗提出了更高的挑战。此外，传统卡介苗(BCG)对儿童和成年人肺结核病的预防效果不稳定，且免疫学作用机制尚不完全清楚，这也导致结核病的预防陷入困境。然而，结核病的发生、发展和转归除了与机体载菌量及其毒力等因素有关，机体的免疫功能也起到了非常重要的作用。因此，充分了解结核分枝杆菌免疫应答与逃逸机制，对结核病的临床诊治、新型结核疫苗与免疫疗法的研究都具有重要的理论指导意义。
[0003]结核分枝杆菌是一种胞内致病菌，主要寄生于巨噬细胞中，其通过多种策略逃避宿主细胞的免疫杀伤作用，从而在在宿主体内缓慢生长并长期存活。这是通过特定病原体蛋白和宿主免疫细胞之间的分子相互作用发生的。由于分子间相互作用会影响分子功能发生改变，从而导致病原体改变其基因表达过程、调控病原体从复制(生长)状态到非复制(休眠)状态之间的转换并激发产生能量的替代机制。因此，这些分子间相互作用对结核分枝杆菌在宿主体内的存活至关重要，它们可以调节宿主对细菌感染的免疫应答并获取其生长所需的营养物质。这表明结核分枝杆菌感染时与宿主相互作用的蛋白，可能是设计新型抗结核药物和疫苗的潜在靶点。

技术实现思路

[0004]为了克服现有技术的不足，本专利技术的第一个目的在于提供一种结核分枝杆菌蛋白组，对于结核病的诊断、治疗，特别是结核分枝杆菌的发病机制和开发结核疫苗的研究提供潜在的靶点。
[0005]本专利技术的第二个目的在于提供一种上述结核分枝杆菌蛋白组的筛选方法，该筛选方法结果客观，效率高且降低了成本，有一定的优越性。
[0006]本专利技术的第三个目的在于提供一种上述结核分枝杆菌蛋白组的应用，为将其用于制备检测试剂、治疗试剂或是用于研究结核分枝杆菌的作用机制，具有良好的辅助开发应用。
[0007]实现本专利技术的第一个目的可以通过采取如下技术方案达到：一种结核分枝杆菌蛋白组，包括Rv3768、Rv1848、Rv0028、Rv0301、Rv1022、Rv0095c、Rv2523c、Rv0546c、Rv1778c、Rv0100、Rv1724c、Rv1875、Rv3735、RV3284、Rv0577、Rv3849、Rv2697c、Rv2360c、Rv0489、Rv3246c、Rv3841、Rv3285、Rv0764c、Rv2837c、Rv2907c、Rv3756c、Rv3118、Rv3291c、Rv2534c、Rv0865、Rv1876、Rv3688c、Rv3295、Rv3283、RV1404、Rv0036、Rv1070c、Rv1719、Rv3855、Rv2731、Rv2324、Rv0815c、Rv2754c、Rv3257c、MT2455、Rv0025、Rv3013、Rv0009、Rv1628c、Rv3420c、Rv2779c、Rv3089、Rv0187、Rv1356c、RV3503C、Rv2528c、MT0946、Rv0130、Rv2098c、Rv0730、Rv0887c、Rv2883c、Rv2564、Rv3042c、Rv3788、Rv2158c、Rv2258c、Rv2102、Rv0398c、Rv0861c、Rv0459、Rv1742、Rv3009c、Rv1284、Rv0384c、Rv1860、Rv0390、Rv2426c、Rv2243、Rv2233、Rv2889c、Rv1654、Rv1094、Rv1692、Rv2043c、Rv1828、Rv3117、Rv1389、Rv2558、Rv0678、Rv3315c、RV2619C、Rv3050c、Rv0333、Rv0793、Rv1059、Rv1405c、Rv3836、Rv1046c、Rv1710、RV2438C、Rv0046c、Rv2460c、Rv1604、Rv0640、Rv0571c、Rv0551c、Rv1657、Rv1794、Rv1142c、Rv3198c、Rv1896c、Rv3279c、Rv1531、Rv2461c、Rv3154、Rv1015c、Rv3007c、Rv2511、Rv3677c、Rv1463、Rv0258c、Rv2492、Rv2614A、Rv1034c、RV2514C、Rv3322c、Rv1112、Rv0952、Rv1919c、Rv1636、Rv0350、Rv0119、Rv0283、Rv2996c、Rv2862c、Rv0984、Rv2680、Rv0351、Rv0310c、MT3289、Rv0396、Rv3160c、Rv1451、Rv0056、MT1560.1、MT3268、Rv3715c、Rv0271c、Rv0511、Rv2478c、Rv1413、Rv2473、Rv2540c、Rv1957、Rv0772、Rv3309c、Rv3301c、Rv2841c、Rv3799c、Rv0684、Rv2114、MT2330.1、Rv2944、RV2026C、Rv2505c、MT1547、RV2467、Rv3395A、Rv1292、RV2282C、Rv1988、Rv1716、Rv3550、Rv2914c、Rv0582、Rv2423、Rv2525c、Rv0078A、Rv2471、Rv0274、Rv2291、Rv3699、Rv1852、Rv1478、Rv2096c、Rv1717、Rv2374c、Rv2521、Rv2851c、Rv3099c、Rv1677、Rv3237c、RV1379、Rv1377c、Rv1347c、Rv0491、Rv2959c、Rv2913c、Rv2718c、Rv1671、Rv1019、Rv1219c、Rv2179c、Rv2185c、RV1949C、Rv2513、Rv2149c、Rv1683、Rv3521、Rv1003、Rv1419、Rv0726c、Rv0970、Rv0078、Rv0821c、Rv2689c、Rv2825c、Rv3442c、Rv2436、Rv3465、Rv3748、Rv0232、Rv2477c、Rv0045c、Rv2376c、Rv331本文档来自技高网...

【技术保护点】

【技术特征摘要】
1.一种结核分枝杆菌蛋白组，其特征在于包括Rv3768、Rv1848、Rv0028、Rv0301、Rv1022、Rv0095c、Rv2523c、Rv0546c、Rv1778c、Rv0100、Rv1724c、Rv1875、Rv3735、RV3284、Rv0577、Rv3849、Rv2697c、Rv2360c、Rv0489、Rv3246c、Rv3841、Rv3285、Rv0764c、Rv2837c、Rv2907c、Rv3756c、Rv3118、Rv3291c、Rv2534c、Rv0865、Rv1876、Rv3688c、Rv3295、Rv3283、RV1404、Rv0036、Rv1070c、Rv1719、Rv3855、Rv2731、Rv2324、Rv0815c、Rv2754c、Rv3257c、MT2455、Rv0025、Rv3013、Rv0009、Rv1628c、Rv3420c、Rv2779c、Rv3089、Rv0187、Rv1356c、RV3503C、Rv2528c、MT0946、Rv0130、Rv2098c、Rv0730、Rv0887c、Rv2883c、Rv2564、Rv3042c、Rv3788、Rv2158c、Rv2258c、Rv2102、Rv0398c、Rv0861c、Rv0459、Rv1742、Rv3009c、Rv1284、Rv0384c、Rv1860、Rv0390、Rv2426c、Rv2243、Rv2233、Rv2889c、Rv1654、Rv1094、Rv1692、Rv2043c、Rv1828、Rv3117、Rv1389、Rv2558、Rv0678、Rv3315c、RV2619C、Rv3050c、Rv0333、Rv0793、Rv1059、Rv1405c、Rv3836、Rv1046c、Rv1710、RV2438C、Rv0046c、Rv2460c、Rv1604、Rv0640、Rv0571c、Rv0551c、Rv1657、Rv1794、Rv1142c、Rv3198c、Rv1896c、Rv3279c、Rv1531、Rv2461c、Rv3154、Rv1015c、Rv3007c、Rv2511、Rv3677c、Rv1463、Rv0258c、Rv2492、Rv2614A、Rv1034c、RV2514C、Rv3322c、Rv1112、Rv0952、Rv1919c、Rv1636、Rv0350、Rv0119、Rv0283、Rv2996c、Rv2862c、Rv0984、Rv2680、Rv0351、Rv0310c、MT3289、Rv0396、Rv3160c、Rv1451、Rv0056、MT1560.1、MT3268、Rv3715c、Rv0271c、Rv0511、Rv2478c、Rv1413、Rv2473、Rv2540c、Rv1957、Rv0772、Rv3309c、Rv3301c、Rv2841c、Rv3799c、Rv0684、Rv2114、MT2330.1、Rv2944、RV2026C、Rv2505c、MT1547、RV2467、Rv3395A、Rv1292、RV2282C、Rv1988、Rv1716、Rv3550、Rv2914c、Rv0582、Rv2423、Rv2525c、Rv0078A、Rv2471、Rv0274、Rv2291、Rv3699、Rv1852、Rv1478、Rv2096c、Rv1717、Rv2374c、Rv2521、Rv2851c、Rv3099c、Rv1677、Rv3237c、RV1379、Rv1377c、Rv1347c、Rv0491、Rv2959c、Rv2913c、Rv2718c、Rv1671、Rv1019、Rv1219c、Rv2179c、Rv2185c、RV1949C、Rv2513、Rv2149c、Rv1683、Rv3521、Rv1003、Rv1419、Rv0726c、Rv0970、Rv0078、Rv0821c、Rv2689c、Rv2825c、Rv3442c、Rv2436、Rv3465、Rv3748、Rv0232、Rv2477c、Rv0045c、Rv2376c、Rv3311、RV0687、Rv0937c、Rv3559c、Rv2847c、Rv2703、Rv3280、Rv1827、Rv3168、RV1013、Rv2499c、RV3213C、Rv0956、Rv0429c、Rv1718、RV0534C、Rv3609c、Rv0041、Rv1056、RV1264、Rv2293c、Rv3899C、Rv3676、Rv3717、Rv2711、Rv3248c、MT3269、Rv1080c、Rv3002c、Rv0191、MT2625、MT1196、Rv2909c、MT3780、Rv1667c、Rv1202、Rv0030、Rv0113、Rv0110、Rv2765、Rv1829、Rv1414、RV1416、Rv1685c、Rv2310、Rv0801、Rv3678A、MT1364、Rv0099、Rv3709c、Rv2202c、Rv1045、Rv1606、Rv2333c、RV1331、Rv3838c、Rv3406、MT3103、Rv2818c、Rv3039c、Rv3672c、Rv1816、Rv0518、Rv1159A、Rv2557、Rv3791、Rv2358、Rv0831c、Rv3567c、Rv3705c、Rv0737、Rv1448c、Rv0957、Rv2546、Rv1147、Rv3592、Rv0146、Rv0158、Rv0076c、Rv1882c、Rv1245c、Rv2238c、Rv3061c、Rv2400c、Rv3561、Rv2626c、Rv0164、Rv0733、Rv0195、MT0085.1、Rv0138、Rv2134c、Rv3557c、Rv0855、Rv0583c、Rv0361、Rv1820、MT2068、Rv3241c、Rv1680、Rv2927c、Rv1725c、Rv2712c、RV3573C、Rv2234、Rv3588c、Rv2498c、Rv0552、Rv0422c、Rv0385、Rv2384、Rv1894c、Rv1428c、Rv2133c、Rv0364、Rv2683、RV1025、Rv3076、Rv0566c、Rv0178、Rv1109c、MT0812、Rv0302、Rv1911c、Rv2368c、Rv0905、Rv2503c、Rv1337、Rv3755c、Rv2763c、Rv3472、RV1837c
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1、Rv3427c、Rv2912c、Rv2299c、Rv2226、Rv1299、Rv2696c、Rv0273c、Rv2407、Rv0857、Rv2115c、Rv2592c、Rv3121、Rv1262c、Rv1932、RV2535C、RV1395、Rv0072、Rv1766、Rv1439c、Rv1536、Rv2495c、Rv0585c、Rv1168c、Rv3835、Rv3341、
MT1342、RV2937、Rv1698、Rv3437、Rv1362c、Rv3024c和Rv0155中的至少一种。2.如权利要求1所述的结核分枝杆菌蛋白组，其特征在于，所述Rv3768、Rv1848、Rv0028、Rv0301、Rv1022、Rv0095c、Rv2523c、Rv0546c、Rv1778c、Rv0100、Rv1724c、Rv1875、Rv3735、RV3284、Rv0577、Rv3849、Rv2697c、Rv2360c、Rv0489、Rv3246c、Rv3841、Rv3285、Rv0764c、Rv2837c、Rv2907c、Rv3756c、Rv3118、Rv3291c、Rv2534c、Rv0865、Rv1876、Rv3688c、Rv3295、Rv3283、RV1404、Rv0036、Rv1070c、Rv1719、Rv3855、Rv2731、Rv2324、Rv0815c、Rv2754c...

【专利技术属性】
技术研发人员：张晨晨，魏文静，董文雅，王雪枝，陈亮，陈珣珣，廖庆华，赵雨川，巫株华，彭柯皓，徐华丽，黄珊珊，
申请(专利权)人：广东省结核病控制中心，
类型：发明
国别省市：