一种通过物理途径促进干细胞分化的材料及方法技术

本发明专利技术涉及一种通过物理途径促进干细胞分化效率的材料及方法，具体公开了一种促进人胚胎干细胞向造血相关细胞亚型分化的颗粒基底材料，所述颗粒基底材料是由微球和纳米球通过自组装在一容器中或基底表面形成；所述的微球与纳米球的粒径尺寸比例为1:0.01

【技术实现步骤摘要】
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【技术保护点】

【技术特征摘要】
1.一种促进干细胞向造血相关细胞分化的颗粒晶体膜，其特征在于，所述颗粒晶体膜由微球和纳米球通过自组装在基底表面形成；所述的微球选自氧化无机微球或聚合物有机微球；所述的纳米球选自表面进行化学修饰的纳米球；所述的微球的粒径为1μm
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6μm；所述的纳米球的粒径选自30nm
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500nm；且微球与纳米球的粒径比例为2:0.03
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0.5；优选地，氧化无机微球选自二氧化硅微球，聚合物有机微球选自聚苯乙烯微球、聚苯乙烯微球、聚甲基丙烯酸甲酯微球；优选地，表面进行化学修饰的纳米球选自表面进行羧基修饰的纳米球，更优选为表面进行羧基修饰的聚苯乙烯纳米球。2.根据权利要求1所述的颗粒晶体膜，其特征在于，所述的微球和纳米球的数量比为1:10000～1:60000。3.一种细胞培养的容器，其特征在于，所述的容器与细胞接触的表面上具有如权利要求1或2所述的颗粒晶体膜；优选地，所述的容器的种类选自细胞培养皿、细胞培养板、细胞爬片。4.一种诱导干细胞向功能细胞分化的方法，其特征在于，所述方法包括以下步骤：1)采用权利要求1或2所述的颗粒晶体膜表面或者采用权利要求3所述的细胞培养的容器，培养干细胞，进行增殖培养；2)诱导步骤1)增殖后的干细胞分化；优选地，所述的功能细胞选自造血细胞、造血干细胞、造血祖细胞、红系细胞中的至少一种。5.一种制备具有诱导干细胞分化的表面的方法，其特征在于，所述方法包括在基底上形成权利要求1或2所述的颗粒晶体膜...

【专利技术属性】
技术研发人员：王鹏元，林姣，陈波，
申请(专利权)人：深圳先进技术研究院，
类型：发明
国别省市：