The invention relates to a sample preservation solution for detection of parasite eggs and the use method of sample preservation solution for detection of parasitic eggs, including malachite green, pH buffer, twelve alkyl sulfonate, polyformaldehyde, methanol, ethanol, glycerin, L and alanine. The beneficial effects of the invention are as follows: (1) the preservation solution of the invention can be applied to the preservation and treatment of human and animal feces, and the cost is low. In the case of keeping the parasite eggs intact, the sample can be preserved for a long time and facilitate the transportation, improve the environment in the detection and keep it in the liquid base cell treatment liquid of the invention. The morphology of the parasitic eggs was well fixed and maintained to avoid the swelling, rupture and atrophy of parasitic eggs and facilitate the diagnosis. (2) the invention is used for sample preservation solution for parasite eggs detection. The components contain components that soften the visual field background and reduce the fatigue of the field of vision, and are convenient for observation.
【技术实现步骤摘要】
一种用于寄生虫卵检测的样品保存液(一)
本专利技术涉及一种用于寄生虫卵检测的样品保存液,以及利用所述用于寄生虫卵检测的样品保存液检测粪便中寄生虫卵的方法。(二)
技术介绍
寄生虫卵侵入宿主,可以产生多种危害而影响宿主的健康。寄生虫卵可以随宿主的粪便排出体外,因此可以通过粪便检测是否感染寄生虫以及感染的程度。检测寄生虫卵常用的方法,如直接检查粪便中的虫体,生理盐水涂片法,加藤法等,但这些方法均受一定条件的限制,如样本不能长期保存,检测时臭味较重,耗时等。(三)
技术实现思路
针对现有粪便寄生虫检测技术的不足,本专利技术的目的是提供一种能够快速检测,较长时间保存粪便样本,并改善检测粪便的环境,降低检测时的臭味的用于寄生虫卵检测的样品保存液,以及利用所述用于寄生虫卵检测的样品保存液检测粪便中寄生虫卵的方法。本专利技术采用的技术方案是:一种用于寄生虫卵检测的样品保存液,主要包括以下组分:多聚甲醛50~100g/L、甲醇10~30g/L、乙醇10~50g/L,L-丙氨酸0.3~0.8g/L、L-异亮氨酸0.3~0.8g/L、磷酸盐缓冲液0.005~0.015mol/L,十二烷基硫酸钠0.1~0.3g/L,香兰素5~10g/L,孔雀石绿0.05~0.09g/L,甘油30~80g/L,调pH6.2~6.8,余量为水。优选的,其组成如下:多聚甲醛60~80g/L、甲醇20~25g/L、乙醇20~30g/L,L-丙氨酸0.5~0.6g/L、L-异亮氨酸0.4~0.6g/L、磷酸盐缓冲液0.008~0.01mol/L,十二烷基硫酸钠0.15~0.2g/L,香兰素6~7g/L,孔雀 ...
【技术保护点】
1.一种用于寄生虫卵检测的样品保存液,主要包括以下组分:多聚甲醛50~100g/L、甲醇10~30mL/L、乙醇10~50mL/L,L‑丙氨酸0.3~0.8g/L、L‑异亮氨酸0.3~0.8g/L、磷酸盐缓冲液0.005~0.015mol/L,十二烷基硫酸钠0.1~0.3g/L,香兰素5~10g/L,孔雀石绿0.05~0.09g/L,甘油30~80g/L,调pH 6.2~6.8,余量为水。
【技术特征摘要】
1.一种用于寄生虫卵检测的样品保存液,主要包括以下组分:多聚甲醛50~100g/L、甲醇10~30mL/L、乙醇10~50mL/L,L-丙氨酸0.3~0.8g/L、L-异亮氨酸0.3~0.8g/L、磷酸盐缓冲液0.005~0.015mol/L,十二烷基硫酸钠0.1~0.3g/L,香兰素5~10g/L,孔雀石绿0.05~0.09g/L,甘油30~80g/L,调pH6.2~6.8,余量为水。2.如权利要求1所述的用于寄生虫卵检测的样品保存液,其特征在于其组成如下:多聚甲醛60~80g/L、甲醇20~25mL/L、乙醇20~30mL/L,L-丙氨酸0.5~0.6g/L、L-异亮氨酸0.4~0.6g/L、磷酸盐缓冲液0.008~0.01m...
【专利技术属性】
技术研发人员:魏兴江,
申请(专利权)人:博朗厦门生物科技有限公司,
类型:发明
国别省市:福建,35
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