The invention belongs to the technical field of plant gene engineering, in particular to a gene for controlling cold tolerance of rice and its application. A gene OsCPK24 induced by cold stress was isolated from rice, and its nucleotide sequence, as shown by SEQ ID NO:1, was shown by the sequence of protein encoded by the gene, such as SEQ ID NO:2. The CDS of OsCPK24 was cloned to pC1300s, and the overexpression vector was obtained. The sequence of SEQ ID NO:3 was cloned to pDS1301, and RNAi inhibition expression vector was obtained, and the OsCPK24 overexpression transgenic line and inhibition expression line were obtained. Under cold stress, the survival rate of the overexpressed transgenic lines was higher than that of the wild type, and the survival rate of the transgenic lines was lower than that of the wild type, indicating that the OsCPK24 gene had the function of regulating the cold tolerance of rice. The invention provides new resources for cold resistant breeding of plants and rice.
【技术实现步骤摘要】
一种控制水稻耐冷性的基因及其应用
本专利技术属于植物基因工程
具体涉及一种控制水稻耐冷性的基因OsCPK24及其应用。
技术介绍
水稻(Oryzasativa)是世界第二大粮食作物，产量约占世界粮食总产的1/3。水稻起源于热带或亚热带地区，属于喜温作物，对低温很敏感。低温冷害是影响水稻稳定生产的主要逆境之一。全球每年约有1500万hm2水稻受到寒冷胁迫(Louetal.,2007.Euphytica158:87-89)，在南亚和东南亚约有700万hm2面积因低温影响不能种植现代水稻品种(Sthapitetal.,1998.CropSci38:660-666)，而我国每年因冷害损失稻谷约50亿kg(潘英华等，2010，中国农学通报，2010，26(17)：54-59)。水稻芽期和苗期冷害多发生在我国长江流域早稻区和云、贵、川等高纬度及高海波一季中稻区，这些稻区在水稻生长前期常遇寒潮侵袭，非耐冷品种幼芽和幼苗停止生长，易导致烂秧、死苗或弱苗、抽穗分散，成熟期不一致等现象，严重影响水稻产量(Andayaetal.,2003.JExpBot54:2579-2585...
【技术保护点】
1.一种分离的蛋白，其蛋白质序列如SEQ ID NO：2所示。
【技术特征摘要】
1.一种分离的蛋白，其蛋白质序列如SEQIDNO：2所示。2.编码权利要求1所述蛋白的基因，其核苷酸序列如SEQIDNO：1中第1-1542位碱基所示的序列。3.一个超量表达载体p1300s-OsCPK24，其特征在于，该载体含有权利要求2所述的基因。4.权利要求1所述的蛋白在调控水稻抗冷性中的应用。5.权利要求2所述的基因在改善水稻耐冷性和提高水稻冷胁迫下产量的应用。6.一种提高植物耐冷性的方法，其特征在...
【专利技术属性】
技术研发人员：林拥军，刘瑜，许纯珏，张利娜，周菲，马伟华，陈浩，
申请(专利权)人：华中农业大学，
类型：发明
国别省市：湖北,42
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