The invention discloses a method for rapid cultivation of the tissue culture seedlings of deciduous holly, including: 1) axillary buds obtained by regular sterilizing and inoculating methods are obtained by the first generation of the axillary buds, and the sterile buds of 1.5~2.0cm are obtained after the first generation, and the sterile buds are transferred into the proliferation medium, and a small amount of bushes are produced first, and then a large number of clusters are obtained by subculture. 2) the growth of the cultured deciduous Holly cluster buds was transferred into the medium of strong seedlings. After 25d, the buds continued to proliferate, and the number of effective seedlings increased; 3) the strong buds of more than 3.0cm were inoculated into the rooting induction medium, and 15~25d began to rooting after 35~45d, and the regenerated plants grew to more than 5.0~6.0cm, small Miao Jian. Zhuang; 4) when the young shoots grow to more than 5.0~6.0cm or 8~10, the seedlings are seeded and domesticated and transplanted. The invention is simple and easy to operate. It needs 75 days to proliferate and grow seedlings, induce the root to advance to 15~25 days, the highest proliferation rate of buds up to 415~446%, the rooting rate of 65~80%, and the survival rate of more than 85%~90%.
【技术实现步骤摘要】
一种快速培育落叶冬青组培苗的方法
本专利技术涉及落叶冬青组培
,具体涉及一种快速培育落叶冬青组培苗的方法。
技术介绍
落叶冬青(IlexverticillataL.)是引自北美地区东部的一种冬青科冬青属的落叶灌木,雌雄异株,通常分布于沼泽与池塘的周围,有许多栽培品种。落叶型冬青与其它常绿冬青树种不同,其特点是冬季落叶,鲜艳饱满的红色果实挂满枝头,挂果期可至翌年4月,给寒冷的严冬带来温暖和喜庆的颜色,是冬季赏果的最佳树种之一。即可用作商业切枝又可做庭院绿化、室内盆景、冬季游园观赏之用,极具园林观赏和商业价值。落叶型冬青引种到我国的时间不长,苗木数量较少。为保持落叶冬青的观赏价值,扩大推广范围,无性繁殖是保持遗传特色的最好方法,而组织培养法是快速获得大量繁殖苗的最佳方法。但由于目前落叶冬青组培苗增殖系数少、生根慢、生根率低,限制了其大规模的推广和繁殖。
技术实现思路
专利技术目的:针对现有技术中繁殖系数和生根率低,生根时间长等存在的不足,本专利技术的目的是提供一种快速培育落叶冬青组培苗的方法,采用组织培养的方法对不同落叶冬青品种的腋芽进行增殖、壮苗、生根培养,建立快速获得再生植株的技术方法,解决了落叶冬青扦插繁殖受季节和种苗限制、组培苗增殖率低、生根慢、生根率低、移栽成活率低等难题,适合多个引种落叶冬青品种,可加快引种落叶冬青的繁殖速度和规模,具有较强的实用性。技术方案:为了实现上述专利技术目的,本专利技术采用的技术方案为:一种快速培育落叶冬青组培苗的方法,其特征在于,包括以下步骤:1)取经常规消毒、接种方法获得的腋芽,进行初代培养,获得1.5~2.0cm的 ...
【技术保护点】
一种快速培育落叶冬青组培苗的方法,其特征在于,包括以下步骤:1)取经常规消毒、接种方法获得的腋芽,进行初代培养,获得1.5~2.0cm的无菌芽;将无菌芽剪切转接入增殖培养基中,先培养产生少量丛生芽,然后以25d为继代周期,将带有5~6个小芽的芽丛再转接入增殖培养基中,获得大量的丛生芽;增殖培养基为:MS+0.5mg/L NAA+0.5mg/L 6‑BA+1.0mg/L CPPU,或MS+0.1mg/L NAA+0.5mg/L 6‑BA+0.5mg/L CPPU +1.0mg/L GA,或MS+0.5 mg/L NAA+0.2 mg/L 6‑BA+0.5 mg/L CPPU;2)将增殖培养后的落叶冬青丛芽转接入壮苗培养基中,培养25d后,丛芽继续增殖,且有效苗数量增加;壮苗培养基为:MS+0.1 mg/L NAA+0.5 mg/L6‑BA+0.5 mg/L CPPU+ 1.0 GA;3)剪取3.0cm以上的壮芽接种到生根诱导培养基中,15~25d后开始生根,培养35~45d后,再生植株长至5.0~6.0cm以上,小苗健壮;生根培养基为:1/2MS+1.0 mg/L IBA+1.0 g/L ...
【技术特征摘要】
1.一种快速培育落叶冬青组培苗的方法,其特征在于,包括以下步骤:1)取经常规消毒、接种方法获得的腋芽,进行初代培养,获得1.5~2.0cm的无菌芽;将无菌芽剪切转接入增殖培养基中,先培养产生少量丛生芽,然后以25d为继代周期,将带有5~6个小芽的芽丛再转接入增殖培养基中,获得大量的丛生芽;增殖培养基为:MS+0.5mg/LNAA+0.5mg/L6-BA+1.0mg/LCPPU,或MS+0.1mg/LNAA+0.5mg/L6-BA+0.5mg/LCPPU+1.0mg/LGA,或MS+0.5mg/LNAA+0.2mg/L6-BA+0.5mg/LCPPU;2)将增殖培养后的落叶冬青丛芽转接入壮苗培养基中,培养25d后,丛芽继续增殖,且有效苗数量增加;壮苗培养基为:MS+0.1mg/LNAA+0.5mg/L6-BA+0.5mg/LCPPU+1.0GA;3)剪取3.0cm以上的壮芽接种到生根诱导培养基中,15~25d后开始生根,培养35~45d后,再生植株长至5.0~6.0cm以上,小苗健壮;生根培养基为:1/2MS+1.0mg/LIBA+1.0g/LAC,或1/2MS+0.1mg/LIBA+1.0g/LAC;4)待生根的小苗长至5.0~6.0cm以上、根8~10条时,将小苗进行炼苗、驯化移栽,成活后获得组培苗。2.根据权利要求1所述的快速培育落叶冬青组培苗的方法,其特征在于:步骤1)中,无菌腋芽是经常规消毒法选取落叶冬青春季嫩稍,剪取1.0cm带芽茎段进行腋芽诱导培养20d,获得的1.5~2.0cm左右的无菌芽。3.根据权利要求1所述的快速培育落叶冬青组培苗的方法,其特征在于:...
【专利技术属性】
技术研发人员:陈颖,陈茜,陈燕琼,袁斌玲,魏子涵,
申请(专利权)人:南京林业大学,
类型:发明
国别省市:江苏,32
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