The invention is a temporary storage box after adding 96-well plate (or octopus) in real-time fluorescence quantitative PCR experiment, and belongs to an experimental tool in the field of molecular biology. In real-time fluorescence quantitative mass detection, many experimenters are accustomed to mixing the common reagents needed for each hole before packing, which can not only reduce the workload, but also make the mixing more fully, with less errors, and add a lot of samples to be tested at a time. However, a real-time fluorescence quantitative PCR instrument usually detects 96 holes in two hours at most, so it often encounters the situation that samples need to wait for machine detection. If 96 orifice plates (or eight connecting tubes) are directly put into a refrigerator at 4 C, the samples have been mixed with fluorescent groups, and others repeatedly open the refrigerator door, the refrigeratory light will affect the accuracy of the experimental data. The six-sided Brown shading treatment of the invention can minimize the influence of the light in the refrigerator on the experimental data, and the bottom of the invention can be put into ice blocks so that the samples can be transported on short distances.
【技术实现步骤摘要】
一种实时荧光定量PCR加样96孔板暂存盒
本专利技术是生物分子水平研究领域,实时荧光定量技术检测样本时,加样样本的暂存盒。
技术介绍
聚合酶链反应(PCR)是生命遗传物质DNA的体外扩增,而实时荧光定量技术,在传统的PCR反应中加入一种荧光基团,利用荧光信号累计来监测整个PCR进程,通过数据分析,实现样本的定量检测,使PCR技术从定性到定量有了质的飞跃。实时荧光定量PCR检测是最近兴起的一种分子水平的检测方法,以其灵敏度高、特异性强、重复性好、速度快、安全可靠、自动化程度高等特点迅速成为分子生物学研究的重要工具。实时荧光定量PCR检测前需要先加样,即每个检测孔均需加入上下游引物、水、模板、荧光基团等。大批量做实时荧光定量PCR检测时(比如检测很多样本的某种病原体、筛选分子水平的诊断生物标志物等),需要加很多样孔,为了加样的方便,很多实验者先把各个孔共用的试剂先混合,再分装,这样不仅极大减小了工作量,而且反应液混合更均匀。但是一台实时荧光定量PCR机一次(大约两个小时)最多检测96个孔的数值,经常遇到加好的样本等待检测的情况。此时样本中已经混合有荧光基团,为了保证实验数据的准确性,设计了该专利技术。
技术实现思路
该专利技术是一个六面均为棕色的PC材料的盒子,外部分盖和体,内部由两隔板分三层,第一层隔板为可以放入96孔板的塑料板,第二层隔板稍低于96孔(八连管)的最底部,第二层隔板下可以加入水或冰袋。放入4℃冰箱,六面棕色设计,可防止其他人反复开冰箱门,冰箱灯亮造成对样本的影响,该盒子第二层隔板下放入冰袋,可以实现样本的较短途运输。附图说明图1.该专利技术整体俯 ...
【技术保护点】
1.该专利技术六面棕色遮光处理。
【技术特征摘要】
1.该发明六面棕色遮光处理。2.该发明外部整体分两部分,盒盖和盒体,盒盖略大于盒体。3.该发明内...
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