The invention belongs to the field of medical inspection technology, and discloses a method for detecting fungi by fluorescent staining, which can be used for collecting samples such as skin scraps, nails, etc.: 1. specimens are placed on slides; 2. dripping solution No. 1; 3. flame fixation; 4. dripping solution No. 3; 4. fluorescent staining solution; (5) capping slides; (5) fluorescent microscopic examination fluid specimens: 1. specimens are placed on slides; 2. dripping solution No. 3. Fluorescence staining solution; 4. Covered glass; 5. Fluorescence microscopy. The results of the pre-detection of about 20,000 specimens show that the detection rate of the method is greatly increased from 30% to 88% to 98% compared with the existing fungi detection methods, and the operation steps are simple, requiring only three steps: fixation, dyeing and fluorescence microscopy, with an average of 2 minutes per step, and the detection time of each specimen only needs about 5 minutes after sampling, which greatly improves the detection rate. The detection rate is reduced and the detection time is shortened.
【技术实现步骤摘要】
一种荧光染色真菌检测方法
本专利技术属于医学检验
,尤其涉及一种荧光染色真菌检测方法。
技术介绍
目前,业内常用的现有技术是这样的:现今真菌引起的感染日渐突出,据WHO统计,能引起人类疾病的真菌约有270余种,根据感染部位又可分为浅表真菌和深部真菌。浅表真菌侵袭皮肤、毛发和指甲,而深部真菌感染心、肺、血液、胃肠等人体各个器官和系统,危及患者生命。快速准确地诊断感染的原因对选择适合治疗至关重要,临床上常用的真菌病诊断方法显微镜直接镜检、真菌培养和组织病理检查等检查方法受限于临床医生送检样本的敏感性、取材者的经验和检验医师的检测水平,且耗时长,不利于病人的治疗,尤其给重症病人的治疗带来了严重的危害。综上所述,现有技术存在的问题是:现有技术中,没有结合荧光染色法检测真菌,没有采用一步染色,造成现有技术操作不简便,检测速度慢,不利于及时诊治。现有技术中,显微镜直接镜检阳性率低,准确率低,背景杂质干扰多;真菌分离培养取材要求高,培养要求高,检测周期长,有假阴性。解决上述技术问题的意义:新型真菌检测方法需要简化操作步骤的同时提高检测效率,而结合荧光法检测真菌操作简便,检测结果快速准确。
技术实现思路
针对现有技术存在的问题,本专利技术提供了一种荧光染色真菌检测方法。本专利技术是这样实现的,一种荧光染色真菌检测方法,所述荧光染色真菌检测方法包括:固定:将皮屑、指甲取材标本滴加1号KOH溶液1-2滴,1-3min后火焰固定;体液标本滴加2号甲醛固定液1-2滴,1-3min;染色:取出避光保存的3号吖啶橙荧光染液,滴加1滴,1min后加盖玻片;荧光镜检:将制备好的玻片置于 ...
【技术保护点】
1.一种荧光染色真菌检测方法,其特征在于,所述荧光染色真菌检测方法包括:固定:将皮屑、指甲取材标本滴加1号KOH溶液1‑2滴,1‑3min后火焰固定;体液标本滴加2号甲醛固定液1‑2滴,1‑3min;染色:取出避光保存的3号吖啶橙荧光染液,滴加1滴,1min后加盖玻片;荧光镜检:将制备好的玻片置于荧光显微镜下进行真菌检测。
【技术特征摘要】
1.一种荧光染色真菌检测方法,其特征在于,所述荧光染色真菌检测方法包括:固定:将皮屑、指甲取材标本滴加1号KOH溶液1-2滴,1-3min后火焰固定;体液标本滴加2号甲醛固定液1-2滴,1-3min;染色:取出避光保存的3号吖啶橙荧光染液,滴加1滴,1min后加盖玻片;荧光镜检:将制备好的玻片置于荧光显微镜下进行真菌检测。2.如权利要求1所述的荧光染色真菌检测方法,其特征在...
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