The invention discloses a method for tissue culture and rapid propagation of Machilus Machilus. The method includes the following steps: (1) the acquisition of aseptic seedlings: the seeds are wrapped in gauze, first washed with water, then soaked with alcohol solution, and then washed by sterile water, then disinfectant, sterile water is washed, and then the residual moisture on the surface of the seed is dried by the filter paper, and then inoculated in a culture bottle containing the basic medium. Culture, the explant cluster buds were obtained; (2) proliferation culture: the explant buds were inoculated in the proliferating medium in step (1), and the seedlings were subcultured every 60d (day), and (3) the root culture: step (2) seedling cutting segments, and then inoculated in rooting medium for rooting culture. The method of the invention has short period of cultivation and is not affected by the season, and the survival rate of the seedlings is improved by making seedlings and transplanting, and the yield of the engraving and moistening Nana is high, and the large-scale application of the engraving and moistening Nana is promoted.
【技术实现步骤摘要】
一种刻节润楠的组培快繁方法
本专利技术属于树木繁殖
,特别涉及一种刻节润楠的组培快繁方法。
技术介绍
刻节润楠(Machiluscicatricosa)为樟科(Lauraceae)润楠属(MachilusNees)常绿乔木,高可达15-20m。刻节润楠树皮灰褐色有显著的鳞片脱落后的疤痕,小枝黑褐色;叶椭圆形,薄革质,叶片背面粉绿色,于近小枝端聚生;侧脈稍纤细,每边12-14条;聚伞花序顶生,果深绿色,长圆形;花期:5-6月;果期:7-10月,是园林绿化的优良乡土树种。但其实生苗在常规育种条件下幼苗变异大,生长速度残差不齐。组织培养繁育技术具有繁殖系数高、繁殖速度快、不受季节影响和易于产业化等优点,为了更好地保护、开发与利用刻节润楠资源,研究刻节润楠的组织培养技术具有重要意义。
技术实现思路
本专利技术的目的在于克服现有技术的缺点与不足,提供一种刻节润楠的组培快繁方法。本专利技术的目的通过下述技术方案实现:一种刻节润楠的组培快繁方法,包括如下步骤:(1)无菌苗的获得:将刻节润楠种子包在纱布中,先用水冲洗,接着用酒精溶液浸泡、无菌水冲洗,然后消毒、无菌水冲洗,再用灭菌滤纸吸干种子表面的残留水分,最后接种于装有基本培养基的培养瓶中进行培养,得到外植体丛生芽;(2)增殖培养:将步骤(1)中得到的外植体丛生芽接种于增殖培养基中进行培养,每60d(天)继代一次,得到幼苗;(3)生根培养:将步骤(2)中得到的幼苗切段,再接种于生根培养基中进行生根培养。步骤(1)中所述的刻节润楠种子优选为饱满的刻节润楠种子。步骤(1)中所述的用水冲洗优选为用自来水进行冲洗。步骤(1)中 ...
【技术保护点】
一种刻节润楠的组培快繁方法,其特征在于,包括如下步骤:(1)无菌苗的获得:将刻节润楠种子包在纱布中,先用水冲洗,接着用酒精溶液浸泡、无菌水冲洗,然后消毒、无菌水冲洗,再用灭菌滤纸吸干种子表面的残留水分,最后接种于装有基本培养基的培养瓶中进行培养,得到外植体丛生芽;(2)增殖培养:将步骤(1)中得到的外植体丛生芽接种于增殖培养基中进行培养,每60d(天)继代一次,得到幼苗;(3)生根培养:将步骤(2)中得到的幼苗切段,再接种于生根培养基中进行生根培养。
【技术特征摘要】
1.一种刻节润楠的组培快繁方法,其特征在于,包括如下步骤:(1)无菌苗的获得:将刻节润楠种子包在纱布中,先用水冲洗,接着用酒精溶液浸泡、无菌水冲洗,然后消毒、无菌水冲洗,再用灭菌滤纸吸干种子表面的残留水分,最后接种于装有基本培养基的培养瓶中进行培养,得到外植体丛生芽;(2)增殖培养:将步骤(1)中得到的外植体丛生芽接种于增殖培养基中进行培养,每60d(天)继代一次,得到幼苗;(3)生根培养:将步骤(2)中得到的幼苗切段,再接种于生根培养基中进行生根培养。2.根据权利要求1所述的刻节润楠的组培快繁方法,其特征在于:步骤(1)中所述的基本培养基为添加0.7%(w/w)琼脂和25%(w/w)蔗糖,pH为5.8的基本培养基;步骤(2)中所述的增殖培养基为:Read+1.0mg/LZT+0.03mg/LIBA+2.5%~3.5%(w/w)蔗糖;步骤(3)中所述的生根培养基的配方为:1/2WPM+1.5mg/LIBA。3.根据权利要求1所述的刻节润楠的组培快繁方法,其特征在于:步骤(1)中所述的基本培养基为添加1/4MS、0.7%(w/w)琼脂和25%(w/w)蔗糖,pH为5.8的基本培养基;步骤(3)中所述的生根培养基的配方为:1/2WPM+1.5mg/LIBA+0.1%(w/w)活性炭。4.根据权利要求1所述的刻节润楠的组培快繁方法,其特征在于:步骤(1)中所述的酒精溶液的浓度为体积百分比75%。5.根据权利要求1所述的刻节润楠的组培快繁方法,其特...
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