The invention provides a method for the determination of standard which accord with the characteristics of traditional Chinese medicine ginseng active ingredient content of superposition. The present invention relates to the field of quality control of traditional Chinese medicine, the method of using ginseng extract as its dilute solution, will be a quantitative study of the target of effective components of standard solution diluted to the concentrations of the standard series solution after the detector to determine the target of effective components standard calibration curve, and the method for content determination of the effective components of the target. This method solves the dilution series of concentration standard solution obtained using the standard calibration curve of pure solvent, without considering the matrix effect, the analyte in the pure solvent and the extraction of traditional Chinese medicine in the detection environment is different, thus affecting the accuracy, reproducibility and selectivity of HPLC method for the determination of the problem. The standard superposition method can maximize the return of effective components of target detection in different environment of the detector, thus deducting interference caused by endogenous substances were eluted on the effective components of the target, effectively eliminate the matrix effect, the content of effective components of ginseng determination result is more accurate.
【技术实现步骤摘要】
一种人参有效成分含量测定方法
本专利技术涉及中药质量控制领域,具体涉及一种适用于人参中有效成分含量测定的方法,该方法以人参自身提取液为稀释溶液,将需定量研究的有效成分标准溶液稀释为系列浓度标准工作溶液后经检测器检测以确定目标分析物标准校正曲线,从而对目标有效成分进行含量测定的方法。
技术介绍
人参皂苷Rb1,中文化学名称:(3β,12β)-20-[(6-O-β-D-吡喃葡萄糖基-β-D吡喃葡萄糖基)氧]-12-羟基达玛脂-24-烯-3-y12-O-β-D-吡喃葡萄糖苷,英文化学名称:(3β,12β)-20-[(6-O-β-D-Glucopyranosyl-β-D-glucopyranosyl)oxy]-12-hydroxydammar-24-en-3-yl2-O-β-D-glucopyranosyl-β-D-glucopyranoside。人参皂苷Rb1存在于五加科植物人参的根、茎和叶中,西洋参的根、茎和叶中,三七的根和叶中,以及葫芦科绞股蓝的叶和茎中,分子式为C54H92O23,相对分子质量为1108.26,白色粉末,易溶于水、甲醇和乙醇,可溶于正丁醇、醋酸和乙酸乙酯,不溶于乙醚和苯,CAS号:41753-43-9。人参皂苷Rg1,人参皂苷Rg1存在于五加科植物人参的根,三七的根,分子式为C42H72O14,相对分子质量为801.01,白色粉末,易溶于水、甲醇、乙醇,不溶于乙醚、苯。CAS号:22427-39-0。人参皂苷Re,人参皂苷Re存在于存在于五加科植物人参的根、花蕾、茎、叶和芦头中,西洋参的根中,分子式为C48H82O18,相对分子质量为947 ...
【技术保护点】
一种用于检测人参中有效成分含量的分析方法,包括以下步骤:(1)精密称取标准品适量,用甲醇溶解后,配制混合标准溶液母液备用;(2)用人参药材提取液将混合标准溶液母液稀释成系列浓度标准工作溶液,取药材提取液作为对照样品;(3)制作标准校正曲线;(4)人参中有效成分含量测定。
【技术特征摘要】
1.一种用于检测人参中有效成分含量的分析方法,包括以下步骤:(1)精密称取标准品适量,用甲醇溶解后,配制混合标准溶液母液备用;(2)用人参药材提取液将混合标准溶液母液稀释成系列浓度标准工作溶液,取药材提取液作为对照样品;(3)制作标准校正曲线;(4)人参中有效成分含量测定。2.如权利要求1所述一种用于检测人参中有效成分含量的分析方法,其中有效成分为人参皂苷...
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